Journal: Insect Science
Article Title: lncR26319/miR‐2834/ EndophilinA axis regulates oogenesis of the silkworm, Bombyx mori
doi: 10.1111/1744-7917.13082
Figure Lengend Snippet: Expression changes of yolk family genes after knockdown of miR‐2834 in BmN4 cells. Expression changes of miR‐2834 (A), lncR26319 (B), and Endophilin A ( EndoA ) (C) after knockdown of miR‐2834. Expression level of yolk protein (Yp) genes were normalized to actinA3 . (D) Expression changes of yolk family genes after repression of miR‐2834. The expression level of yolk family genes was normalized to RP49 . Three independent experiments were performed, and each sample was repeated 3 times (values are means ± SEM of 3 experiments. n.s. P > 0.05, * P < 0.05, *** P < 0.001). Significance analysis was conducted with ANOVA and Student's t ‐test. ANOVA, analysis of variance; SEM, standard error of the mean.
Article Snippet: The lncR26319 ASO was transfected into the BmN4 cells with liposome (FuGENE ® HD Transfection Reagent, Promega, Madison, WI, USA).
Techniques: Expressing, Knockdown